Vol. 25 (64), № 3. 2012
Influence of complex fertilizers on performance structural analysis of winter wheat
Investigated the influence of the modern complex fertilizers company «Valagro» on indicators of structural analysis of plants of winter wheat.
Gender differences of L-arginin metabolism of atletes
Is investigated 26 athletes of both sexes. Which are highly qualified, adapted to long-term performance at heavy physical work of high intensity. Shown gender differences in oxidative and non-oxidative metabolism L-arginine, including it in the nitric oxide synthesis system (oxidative de novo and non-oxidative reutilization routes of NO synthesis), at different stages of their competitive activities. At the stage of maximum functional abilities and adaptation (early competitive period) among girls prevailed non-oxidative metabolism of arginine
with arginase over the oxidative degradation of L-arginine with NO-synthase. During reduction functional abilities (dysadaptation of secondary and final period of competition) the required level of nitric oxide synthesis provided for girls mainly due to resynthesis of nitric oxide from its stable metabolites, whereas for boys – by increasing oxidative de novo synthesis of nitric oxide (as constitutive and inducible).
Virych P.A., Makoveychuck T.I., Schwartau V.V.
Influence of trinexapac-ethyl on content anions in plants of Hordeum vulgare L.
The effect of different concentrations trinexapac-ethyl (TE, Moddus) on the content of free anions (F- , Cl- , NO3- , PO43-, SO42- ) in flag-leaf of plants barley (Hordeum vulgare L.) was studied. Processing plant retardant TE on plants in tillering phase contributed to better ensure the photosynthetic parts of the plant nutrients. TE can play an important role in the development of mineral nutrition systems of plants.
Danilova A.O., Zaporozchenko A.V., Bolshakov L.L.
Study of the effect of drugs with probiotic microorganisms on morphometric parameters of the large intestine, liver and pancreas of rats with alloxan diabetes
The effect of drugs with probiotic microorganisms, which was immobilized on a high carbohydrate preparations, on morphometric parameters of the large intestine, liver, pancreas of rats with induced alloxan diabetes have been studied. It was found that the studied drugs in conditions of alloxan diabetes, which is accompanied by a loss of β-cells of the islets of Langerhans in the pancreas, positive impact on the regeneration of its endocrine and contribute to the stimulation of islet neogenesis, including through the creation of focal zones. Receiving high carbohydrate preparations with probiotic microorganisms improves the colon and reduces the toxic effect of alloxan on the liver cells.
The state of hemoglobin’s system in erythrocytes of patients with erythroemia
It has been shown, that rising of the content of total hemoglobin in erythrocytes of patients with erythroemia is connected with essential increase of the level of methemoglobin (347,2% higher than in control group). At the same time, the percentage part of glycosilated hemoglobin relatively to methemoglobin in erythrocytes of ills was 403,2 percentage less as compare with control group.
Zilbervarg I.R., Zilbervarg E.V., Peskovets S.А.
Landscaping project proposal on terraced slope in the parterre square Culture Palace of Trade Unions in Simferopol
In article gave characteristic of spatial organization, presented the results of the inventory areas, and developed recommendations for planting terraced slope in the parterre Square Trade Unions Palace of Culture in Simferopol.
Kmetko I.L., Sobolev V.I.
The functional condition of the skeletal muscle of white rats in dynamics of the development of experimental hyperthyreosis
In experiments in situ it has been established, that the latent period of the generation of "M-answer" of a forward tibial muscle of white rats has been undergone a number of independent phases in a process of the development of experimental hyperthyreosis: shortenings, increase and stabilization. The latent period of the generation of "M-answer" had been shortened for 15,6% in comparison with control in an initial phase of the formation of hyperthyreosis, and, on the contrary, had been extended for 93% at the expressed degree of hyperthyreosis.
Kobylnik S.N., Razumnova A.Yu., Petrov S.A.
The study of the balance of thiamine in the muscle after allotransplantation of a donor fetal tissue
One of the important trends in modern theoretical and Medical Biochemistry is the study of biochemical processes in the transplantation of fetal tissue to stimulate and restore functions. The aim of our study was to investigate the distribution of thiamine in allotransplantation formed in the embryo-tional and muscle tissue. Transplantation of the abdominal fetal tissue leads to increased levels of free thiamine in the tissues of the acceptor due to reduction in the tissues of the embryo. Transplantation of fetal tissue leads to a decrease in levels of free thiamine in the abdominal muscle of the acceptor. Transplantation of tissues of rats per litter reduces the levels of free thiamine in all tissues examined.
The first data about species composition of spiders (Arachnida, Aranei) in Kazantip Nature Reserve (Crimea)
There are 39 spider species recorded from the Kazantip Nature Reserve. Ero flammeola Simon, 1881 (Mimetidae) is the new record for the former Soviet Union, Ukraine and Crimea.
Korolyeva A.V., Simchuk A.P.
The changeable of RAPD-PCR specters DNA of Scorpaena porcus in the Sevastopol’s bays with different degree of anthropogenic pollution
The investigations of DNA-spectres from Scorpaena porcus’ nervous tissue were made. The DNA-spectres’ peculiarities of Scorpaena porcus from two bays, which have different level of environment pollution, were ascertained. The uncial DNA-fragments of specieses, which are living in more polluted aquatory, were discjvered.The DNA-spectres of Scorpaena porcus, which were gathered from more polluted bay, are characterizing of more degree of uniformity, that is testify about hard nature selection.
Seasonal and infradian rhythmicity nociception of intact snails Helix albescens
Seasonal and infradian rhythmicity of nociceptive sensitivity snails Helix albescens was detected. The highest values of n and winter lower values of the studied parameters were recorded than in the summer, but a few more resistant than in spring. Multi-day rhythmicity indicators of nociception is included rhythms of between ≈2,38 d – ≈9,50 d . An interconnection between seasonal and multi-day rhythmicities was manifested in the difference in the composition of the spectra of infradian rhythmicity and amplitude of rhythms. The smallest number of allocated periods was noted in the summer (n=5), when were registered maximum amplitudes.
The influence of Bacillus thuringiensis on the quantity of the phylloplana epiphytic microflorae and its fungustatic action against the activators of illnesses in the potato agrocenosis
The several strains of B. thuringiensis were selected from the crimean insect populations. The strain B. thuringiensis var. thuringiensis 0371, isolated from Hyphantria cunea Drury caterpillars showed the most insecticidal activity. While processing the potato leaves with bacterial culture we observed the 100% mortality of potato beetle larvae for 5 days. The strain is a technological one and it can be the base for biopreparation for plant protection against leaf-cutting depredators.
Kuleshova D.K.,Davydov V.V.
The activity of blood enzymes of endogenous aldehydes utization in adolescents with a complicated insulinе resistance obesity
The present study was performed to educidate the features of neuroendocrine obesity formation in puberty. The activity of some enzymes of endogenous aldehydes catabolism has been investigated in the blood of adolescents with neuroendocrine obesity, complicated with insulin resistance, or without such a complication. In adolescents with neuroendocrine obesity but without insuline resistance complication in early or late puberty there were observed no blood changes as regards basal power of the enzymatic system of the endogenous aldehydes catabolism. However, in adolescents with a complicated insuline resistance neuroendocrine obesity an adaptive increase in the enzymatic activity of the endogenous aldehydes catabolism was relevated mostly in late puberty. The consequence of such an increase is a decreased blood content of carbonylated products of free radical oxidation in adolescents, aged 16-18, with a complicated insulin resistance obesity.
Kulibaba E.V., Razumnova A.Yu., Petrov S.A.
Study of transaminase activity and protein content in allotransplantation embryonic and formed muscle tissue
The article presents the results of research activity of enzymes and of changes in protein content in muscle tissue at allotransplantation homologous embryonic tissues. Found that transplantation of embryonic muscle tissue to the adult individual does not change transaminaznoy system in muscle tissues
Morphometric characteristic of shrimps Palaemon adspersus and Palaemon elegans (Palaemonidae) from the black sea waters of south-west Crimea (Kaya-Bash and Balaklava Bay)
The shrimp of Palaemon elegans species (Palaemonidae) was found in 2010 – 2011 in the waters of KayaBash. P. adspersus and P. elegans were found in Balaklava Bay. The number of P. adspersus was more than 90% of the sample. The morphometric characteristic of shrimps was presented. The development of larvae P. elegans was studied, their size and description of the larval development stages was given.
Matsyura O.V.,Matsyura M.V.
The structure of the species diversity of island bird communities of Sivash area
The analysis of the island birds’ communities was performed using the key ecological parameters - the index of species diversity, dominance, and evenness. This revealed the differences in communities structure due to their qualitative and quantitative composition. The differences in the spatial structure of island bird communities were determined by the number of dominant species and their significance within the communities. In island systems, where the area suitable for nesting are limited, and territorial competition increases, the dominant species determine the species diversity of bird communities at large extend.
Mikhalskaya L.М. Shvartau V.V., Omelchuk S.T.
Pinoxaden metabolism in winter wheat
Found that the use graminicide Axial 045 EC k.e. in compositions with herbicide Derby 175 SC s.c. and urea after germination did not enhance major pinoxaden residual metabolites M02, M04, M06 and M10 in winter wheat grain.
Muravjova I.P., Mironova T.O.
Lipid-hudrocarbon composinion of Mytilus galloprovincialis (Lamarck, 1819) from hudrotechnical constructions of the Artilleriskaya Bay (Sevastopol, the Black Sea)
It was estimated for the first time that mussel 30 - 40 mm long contains about 2 mg of oil hydrocarbons, while the mussel, which is less than 10 mm contains about 0.2 mg of oil hydrocarbons. Studies have resulted that the mussels from the most contaminated areas of the bay contain the increased amount of lipids and hydrocarbons.The results obtained can be used in monitoring researches.
Orishaka О. V., Vovchuk I.L., Kucherov V.А., Petrov S.А.
Extracting and identification of thiamine pyrophopshokinase from tumour myometrium tissue
Protein spectrum and molecular forms variety of thiamine pyrophopshokinase from the nonmalignant and tumour myometrium tissues were studied by the method of polyacrylamide gel electrophoresis. A method of extracting thiamine pyrophopshokinase isoenzymes and their identification in gel blocs was elaborated. Electrophoretic characteristics of the identified isoenzymes were studied. It has been shown that malignization process is accompanied by changes in protein and enzyme spectra.
Pismenetskaya I.U., Butters T.D.
A structure prediction of blood plasma free oligosaccharides of practically healthy donors
Free oligosaccharides (FOS) are glycans unbound to glycoconjugates, the products of their synthesis and degradation. An accurate analysis of glycan structures is extremely complex because of their enormous diversity. This article is devoted to exploring the possibility of using the information on intracellular FOS and electronic databases on glycans of glycoconjugates to predict blood plasma FOS structures. The chromatographic profiles of plasma FOS of healthy donors obtained experimentally were compared with the structures of glycans and their profiles shown in scientific publications and electronic databases GlycoBase and EUROCarbDB. This approach allowed us to offer the possible structures of carbohydrates for the main peaks of the chromatographic spectrum from blood plasma to develop the ways to further experimental analysis.
The role of nitric oxide in the implementation by microvascular effects of millimeter radiation low intensity
The article is considered the impact of low-intensity millimeter radiation on the system of nitric oxide synthesis. Correlation analysis of microcirculation parameters and nitric oxide showed that the 10-fold effect by milimetric radiation low intensity leads to the optimization of the functional activity of nitric oxide synthesis by the use of the pool circulating of nitrite anion in blood to nitric oxide.
The microelements content in the leaves of pubescent oak influence on the composition of microcommunity phytophagous insects
There is results of study of the microelement content (Cu, Zn, Bb, Cd) in the leaves of pubescent oak trees influence on the composition microcommunities herbivorous insects inhabiting these plants. Found that the density seven of the thirteen species of phytophagous insects spring-summer complex is significantly correlated with the content of heavy metals in the leaves.
Sandetskaya N.V., Kamenchuk O.P., Sitar O.V.
The effect of mineral nutrition conditions on antioxidant status of winter wheat plants parameters
The activity of glutathione reductase, glutathione peroxidase, superoxide dismutase and malondialdehyde content and SH-groups in leaves and roots of wheat seedlings under the actions of foliar treatment by various concentrations of sulfur and phosphorus has been studied. Stated сhanges in antioxidant status of wheat plants under foliar treatment by various concentrations of sulfur and phosphorus was discussed.
Skamrova G.B., Evstigneev M.P., Trushkin A.N., Shckorbatov Yu.G.
The effect of mobile phone and WiMAX network microwave radiation on membrane permeability of human buccal epithelium cells
The effect of low-intensity electromagnetic radiation at 8 GHz frequency, and the influence of electrical and magnetic radiation components separately on membrane permeability and chromatin state in the nuclei of human buccal epithelium cells was investigated. The statistically meaningful cells response to this radiation manifested by increase in the number of heterochromatin granules and membrane permeability with respect to control mean was observed. The electrical component of electromagnetic radiation has the greatest influence on the heterochromatin formation.
Character of dependence of the thermal cost muscular contraction from level of circulatory free triiodothyronine for white rats at experimental thyrotoxicosis
In experiments it is shown on white rats, that at an euthyroidism between the level of free triiodothyronine and thermal cost of muscular contraction there is positive connection (R = 0,82). At an experimental thyrotoxicosis connection (R = 0,88) acquires negative character. It is set that on the high bound of physiological level of triiodothyronine (5, 0 ч 7,1 pmol/l) the thermal cost of unit of work of muscle on 37% is higher, than at the beginning of scale of concentrations of hormone (2, 2 ч 4,8 pmol/l), and at an experimental thyrotoxicosis, the thermal cost of muscular contraction below, than in the initial range of level of triiodothyronine (8 on 19%, 8 ч 11,8 pmol/l). It is shown that precision of process of thermal cost regulation at the state of euthyroidism and experimental thyrotoxicosis gets worse at the height of level of triiodothyronine (accordingly in 2,9 and 2,2 time).
Tokarchuk K.O., Parilova O.O.
Comparative study of acrolein and formaldehyde cytototxic effect on rat thymocytes in vitro
It has been investigated the acrolein (ACR: 5-15 µM) and formaldehyde (FA: 100-300 µM) cytotoxic effect on rat thymocytes, and also their mutual action during 12 and 15 hrs of incubation. The obtained result indicates that LD50 for ACR is 50 µM and for FA – 400 µM. The difference of the cytotoxic action of these aldehydes is that the maximum increment of dead cells is observed for the ACR at 5-10 µM concentration, and for FA – at 0-100 µM. For ACR: at concentration of 0-5 μM increment of dead thymocytes is 0,1 %/ μM, at 5- 10 μM – 0,7 %/μM, at 10-15 μM – 1 %/μM, for the FA: at 0-100 μM – 1 %/μM, at 100-200 μM - 0,1 %/μM, at 200-300 μM – 0,4 %/μM. After 12 hours of incubation with ACR during additional 3 hours the dead cells percentage was same, but with FA this index increased in 1,4 times. Under the ACR and FA mutual action was the significant enhancement of the cytotoxic effect. So, for 12 hours of incubation, the result of their combined effect was greater than mathematical sum in approximately 1,5 times. Therefore, it can be assumed that the development of pathological conditions with hyperproduction of these aldehydes may result in the cytotoxic effects increasing
Tumanyants K.N., Yarmolyuk N.S.
Influence of weak electromagnetic fields of extreme frequency ranges on dynamics and infradian rhythmics of regeneration at planary Dugesia tigrina
It is shown that low-intensive electromagnetic factors of extreme frequency ranges stimulate regeneration of planarian, change infradian rhythmics of their regeneratorny processes. Extent of stimulation of regeneratorny processes at planarian weak electromagnetic radiations of extreme frequency ranges isn't identical. The increase in an index of regeneration, speed of movement at planarian treated to action of a variation magnetic field by frequency of 8 Hz by an induction 50 nTl, than electromagnetic radiation in length of a wave 7,1 mm is more expressed. Changes of rhythmics of regeneratorny processes lasting many days under the influence of EM of factors consists in reorganization of structure of ranges, peak and phase relationship. Electromagnetic radiation of the highest frequency causes more expressed changes of infradian rhythmics of regeneratorny processes in planarian, than a variation magnetic field of ultralow frequency. Distinctions in biological action of the studied factors are found can be connected with change of properties of water under their influence.
Chuyan E.N., Drevetnyak N.A., Bogdanova O.D., Ravaeva M.Yu., Tribrat N.S.
Individually-tipological approach to research of processes of microblood circulation
By a laser Doppler flowmetry (LDF) was studied individually-typological features of indicators of the blood microcirculation parameters in rats. There were 3 types LDF-grammes revealed as a result of research: aperiodic, monotonous with low and high perfusion. The analysis of the micro-blood flow fluxmotions and the parameters of the thermal probe showed the highest equation of the regulatory processes of the microcirculation in aperiodic type LDF-grammes.
Chuyan E.N., Nikiforov I.R., Birukova E.A.
Heart rate variability at examinees in regenerative period after bicycle ergometric test under the electromagnetic radiation extremely high frequency
Heart rate variability within 30-minutes regenerative period after the electromagnetic radiation (EMR), extremely high frequency (EHF) influence.. It is shown that ten-day EMR EHF to increase in adaptive capability of an organism, optimization of regulating mechanisms, decreasing in power expenses and increasing of regenerative speed processes at examinees in answer to the submaximum physical activity.
Yantsev A.V., Kirillova A.V. , Panova S.A., Maidanskaya О.D.
Study effect of essential oil of cedar on the cardiovascular system
Found a statistically significant hypotensive effect of vapours of cedar oil. It was noted a decrease in systolic and diastolic blood pressure. It was also revealed a statistically significant reduction in the frequency of cardiac contractions during the procedures, aromatherapy.
Valiev E.V., Sheykh-Zade M.I.
The application of factor analysis to identify experimental conditions that minimize the relative error in determining the equilibrium constant for the formation of complexes with hydrogen bond.
Obtained the mathematical model of the influence of relationship γ=С0в /С0а between initial concentrations of proton donor С0а and acceptor С0в, K and С0а on the relative error εК of the complexes with hydrogen bond formation process equilibrium constant K in the absence of the proton donor self-association. Based on the analysis of these models revealed the most significant factors affecting theεК. Defined γ values range minimizing the amount εК.
Problems of allocation and clearing plant peroxidase
In the article analysis of existing plant peroxidases isolation and purification methods indicated that none of them is universal. Results of the studies confirm the influence of extractant nature on the catalytic activity of radish black peroxidase.
Gusev A.N., Shulgin V.F., Meshkova S.B.
The influence of size factors on the photoluminescence of zinc complex with 3-(pyridin-2-yl) – 5-(2-salycilideniminophenyl)-1H-1,2,4-triazole.
The factors determining the photoluminescence of zinc complex with 3-(pyridin-2-yl) – 5-(2- salycilideniminophenyl)-1H-1,2,4-triazole. It was found that the photoluminescence of the complex in the solid state depends on the grain size and the degree of crystallinity of the sample. It was shown that the aggregation of the suspension of the complex in DMSO-water leads to a significant increase in the intensity of luminescence.
Comparative characteristics of some methods for the reproducibility estimation of comleximetric titration curve parameters
The paper deals with the comparison of to "th estimations of a titration curve parameters correlating well in the majority of titrations. At unique titration generally the methods based on "the law of distribution of errors" have advantage.
Lekar A.V., Yakovishin L.A., Borisenko S.N., Vetrova E.V., Borisenko N.I., Grishkovets V.I.
Electrospray ionization mass spectrometry of mixtures of triterpene glycosides with acetylsalicylic acid (aspirin)
Using electrospray ionization mass spectrometry, the molecular complexation of acetylsalicylic acid (aspirin) with ivy triterpene glycosides α-hederin (hederagenin 3-O-α-L-rhamnopyranosyl-(1→2)-O-α-Larabinopyranoside) and hederasaponin C (hederagenin 3-O-α-L-rhamnopyranosyl-(1→2)-O-α-Larabinopyranosyl-28-О-α-L-rhamnopyranosyl-(1→4)-О-β-D-glucopyranosyl-(1→6)-О-β-D-glucopyranoside) was for the first time investigated. The glycosides form complexes with acetylsalicylic acid in the 1:1 molar proportion. The complex of acetylsalicylic acid with α-hederin is more stable.
Nіkіpelova O.M., Gorbach L.P., Nіkolenko S.І., Aleksєєnko N.O.
Complete preclinical researchеs of therapeutic muds (peloids) Lake Dzharylgach Crime
Complete preclinical researchеs of therapeutic muds (peloids) Lake Dzharylgach Crimea justified their conditioning, security and the possibility of use in medical practice.
Biocomposites of iodine with polysaccharides
During the work it was found, that iodine have influence on conformation of macromolecule of pectin and alginate. Iodine is formed with an alginate the complex of red-orange color, and with a pectin two types of complexes: red-orange and violet.
Panova E.P., Katseva G.N.
Nanocompos on basis of silver and pectin substances
The worked out method of preparation of nanocomposites of silver where as a reductive-stabilizer agent of nanoparticle the apple and citrus pectin’s are used is the simple and technological method of receipt of unique nanosize materials of wide action spectrum.
Cathodes and anodes discrimination materials for electrochemical synthesis of titanium sulfate(III)
The series of materials for production of cathodes and anodes used in sulfuric acid solutions with a view to identifying current density, stability, toxicity, as well as their value. Analysis is made according to the surge of hydrogen and oxygen in 1.0 mole/dm 3 sulphuric acid solutions, as well as the electrosynthesis titanium sulfate(III) current density 5,0 A/dm 2 and 3,5-4,0 V. Found that the optimum cathode materials are stainless steel, niob energy of electrochemical system.